Scanning Probe Microscopy Overview
Description:
In scanning probe microscopy (SPM), commonly termed atomic force microscopy (AFM), the interaction of a stylus probe and sample surface is quantified and mapped across the sample. The probe or "tip" is of nanometer-scale sharpness, and the standard image is 3D surface topography at a resolution approaching the atomic or molecular scale. The tip is attached to a microfabricated cantilever of low spring constant. Property-sensitive imaging modes are performed simultaneous to topographic imaging. Gaseous or liquid media, plus sample temperature, can be controlled. Tip chemistry can be modified for controlled studies of probe-sample interaction.
The Characterization Facility has four scanning probe microscopes:
- Digital Instruments Nanoscope III Multimode
- Digital Instruments Nanoscope III plus Hysitron TriboScope
- Molecular Imaging PicoScan/PicoPlus
- Molecular Imaging PicoScan/PicoSPM
Accessories common to all SPM instruments:
- Optical access. Ability to acquire digital video image (except PicoSPM).
- Cryo-microtome for cross sectioning samples. Double-D clamp available for mounting cross-sectioned samples in AFM.
- BNC breakout box: standard or available for use.
- Custom adder circuit box for signal manipulation, for example to ramp set point during imaging
- Analog Witec pulsed force mode for adhesion and stiffness imaging (except PicoPlus).
- Infinitesima ActuResonance controller for manipulating the or quality factor (Q) of cantilever resonance.
- Mathematica and ICAdams available for quantitative analysis and modeling of force-distance data and dynamic tip-sample interactions, in order to extract sample storage/loss moduli, surface energy, etc.
- SPIP available for broad suite of image (e.g. grain size) and force curve (e.g. freely jointed chain model) analysis applications. Freeware programs WSXM and Gwyddion also available.
- XYZ manipulator to attach SiO2 or polystyrene microspheres to tipless cantilevers
- Plasma chamber and metal evaporator for chemical modification of tips by users. Tip modification services available, using silane linkage procedures customized by staff member Dr. Jinping Dong.
Applications:
- Sensitive to the following properties: surface chemistry, storage/loss modulus, hardness, interfacial energy, crystallinity, polarization, magnetization, surface charge, and local work function (surface potential).
- Applicable to conductive and non-conductive samples.
- Samples can be imaged in air or in liquid media in all modes of operation.
- Can measure tribological response versus load, scan velocity, temperature, relative humidity.
Capabilities:
- No sample pre-treatment needed and imaging can be performed in air.
- Sample can be conductive or nonconductive, hard or soft.
- Positioning resolution is 0.1 nm laterally and 0.01 nm vertically; imaging lateral resolution depends on sample/tip characteristics (adhesive contact mechanics) and typically is of order 1-10 nm.
Sample Images:


