News and Information
Tecnai Spirit BioTWIN with iCorr
The Tecnai Spirit BioTWIN in Moos Tower now features "iCorr" -- the first
integrated light and electron microscope that combines a wide-field fluorescence
light microscope and electron microscope into a single, harmonized instrument,
thereby enabling a faster more accurate approach to correlative microscopy.
Electron microscopy (EM) offers extremely high image resolution--1,000
or more times better than light microscopes. Light microscopes (LM) offer a
wide variety of specialized techniques, such as fluorescent labeling, that can
identify a single molecule or structure within a large, complex environment
like the interior of a living cell. Correlating between the two techniques
allows scientists to use LM to find the target and EM to explore its form and
function. Correlative microscopy allows investigators to find a feature of
interest faster in the EM, gain additional information about its structure
down to the molecular scale, and understand relationships between the feature
and its larger scale context.
for more information and use of this capability.
FEI Titan Transmission Electron Microscope
We are excited to announce the installation of a new FEI Titan aberration-corrected TEM, as funded by the I3 internal grant competition along with matching funds (lead PI Andre Mkhoyan). This addition takes the University of Minnesota into a new realm of high-resolution characterization, both in imaging and compositional analysis. To accommodate this instrument, two rooms were combined into one and the ceiling was locally raised; a separate walled-in remote operating space was created for cases when greater instrument isolation is needed.
Technical details and capabilities of this instrument include the ability to operate at accelerating voltages ranging from 60 to 300 kV and a spherical-aberration corrector for the probe-forming lenses, yielding a STEM spatial resolution of less than 0.08 nm. The combination of an ultra-bright X-FEG source and the Super-X energy-dispersive X-ray detector system will allow elemental composition maps to be collected approximately 10 times faster than with previous systems. An electron beam monochromator and Gatan Enfinium spectrometer will enable electron energy-loss spectroscopy to be performed with an energy resolution of 0.1 eV, allowing unparalleled access to structural and bonding information and the capability to determine elemental composition at atomic resolution.
The growth and modernization of our TEM enterprise also leads to a heightened need for equipment to handle specimen and specimen holder cleaning for high-resolution work. As announced in our last newsletter, a grant-in-aid award to Prof. Mkhoyan has enabled the purchase of an advanced plasma cleaner for transmission electron microscopy specimens and specimen holders. This augments a 10-year old system that has experienced substantial downtime with increasing age. The new system will provide greater capacity and the additional ability to mix gases for more precisely controlled chemical action. Please contact Jason Myers for more information about the new systems and/or to discuss your TEM needs.