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Visible Light Microscopy

Video-Enhanced Microscope (VEM)


Location:  29 Shepherd Labs

Contacts:  John Nelson

Description:

Visible light microscopes for observing any samples, transparent or opaque, at magnifications up to 4000 x.  Also equipped with video cameras to capture dynamic events, such as emulsification processes or sample change due to temperature.  Uses a PC-based image processing system for image acquisition, processing, and analysis.

Two Nikon Optiphot-Pol microscopes for transmitted and reflected light studies.  Polarized light, phase contrast,dark field,fluorescence, and Nomarski differential interference contrast (DIC) illumination are available.  A monochrome or color video camera relays the microscope image to a computer image processor for background subtraction, contrast enhancement, filtering, and other processing options.

The Metamorph® Imaging System from Universal Imaging Corporation is a windows-based image acquisition, enhancement, analysis, and processing system.  It facilitates particle sizing, data storage, time-lapse image collection, and arithmetic and morphological operations.  Images can be stored on videotape or digitally to the computer.

Specifications:

  • 0.2µm ultimate resolution
  • measurement of objects as small as 0.4µm

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